Development of a QuEChERs Method for Determination of 20 Perfluorinated Compounds in Animal Liver by HPLC_MS/MS
- VernacularTitle:高效液相色谱_串联质谱法测定动物肝脏中20种全氟烷基类化合物
- Author:
Jianli HE
;
Tao PENG
;
Jie XIE
;
Hanhui DAI
;
Dongdong CHEN
;
Zhenfeng YUE
;
Chunlin FAN
;
Cun LI
- Publication Type:Journal Article
- Keywords:
Perfluorinated alkyl substances;
QuEChERs;
High performance liquid chromatography_tandem mass spectrometry;
Animal liver
- From:
Chinese Journal of Analytical Chemistry
2015;(1):40-48
- CountryChina
- Language:Chinese
-
Abstract:
A method for simultaneous determination of 20 Perfluorinated alkyl substances ( PFAS) in animal liver using QuEChERs and HPLC_MS/MS technique was developed. The samples were extracted with 0. 1%hydrochloric acetonitrile and cleaned up with C18 , N_Propylethylendiamine ( PSA ) and graphitized carbon blacks ( GCB ) . The analytes were separated by a reversed phase C18 column and gradiently eluted with a mixed solution of 5 mmol/L ammonium acetate methanol and 5 mmol/L ammonium acetate. The samples were quantified using isotope internal standard and external standard with the matrix matched standard calibration curve method. Good linearity was obtained for all the 20 PFAS at the concentration of 0. 1-10 μg/L with the linear correlation coefficients more than 0. 9995. The limits of detection (LOD) and the limits of quantification ( LOQ) for PFAS were 0. 05-0. 2 μg/kg and 0. 4-0. 5 μg/kg, respectively. The recoveries at three different concentration levels ( 0 . 5 , 2 and 5 μg/kg ) were in the range of 70 . 3% -108 . 1%. The repeatability expressed as relative standard deviations (RSD) was ranged from 2. 1% to 11. 9% (n=6).
