Two Hours Effects of Cyclosporin A on Lymphocyte Subsets in the Body
- VernacularTitle:环孢菌素A体内作用2h对淋巴细胞亚群的影响
- Author:
Zhengjun HU
- Publication Type:Journal Article
- Keywords:
lymphocyte subsets;
cyclosporin A;
hematopoietic stem cel transplantation
- From:
Journal of Zhejiang Chinese Medical University
2014;(11):1308-1310
- CountryChina
- Language:Chinese
-
Abstract:
Objective] By detecting the peripheral blood lymphocyte subsets of AML patients before and after taking cyclosporin A, preliminary discussion on making two hours effects of cyclosporin A on lymphocyte subsets in the body. [Methods] Fasting and taking cyclosporin A two hours of the expression of peripheral blood T, B lymphocytes and NK cel were detected by flow cytometry in 43 cases of patients with AML after transplantation. At the same time, application of chemiluminescence detection concentration of cyclosporin A. [Results] The results of cyclosporin A concentration: Fasting group is 125.43 ±65.94 ng·ml-1. Taking two hours group is 564.46 ±258.67 ng·ml-1; Taking two hours group compared with the fasting group, CD3+ cel is significantly reduced and NK cel is significantly increased, but CD4+, CD8+, CD19+cel and CD4+/CD8+ have no obvious change. [Conclusions] Taking cyclosporin A 2 hours can achieve distribution and affect the number of CD3+ cel in the body, but can not affect the B cel . To AML patients after transplantation, in order to achieve the appropriate concentration and keep abreast of the body's immune status of patients, it is necessary to monitor the concentration of cyclosporin A and lymphocyte subsets during their taking cyclosporin A, which helps us determine the prognosis.
