The effects of morphine combined with cisplatin on invasionon and migration of human lung adenocarci-noma cell A549
- VernacularTitle:吗啡联合顺铂对人肺腺癌细胞 A549侵袭和迁移的影响
- Author:
Ming XIA
;
Jianhua TONG
;
Dapeng GAO
;
Jianguo XU
- Publication Type:Journal Article
- Keywords:
Morphine;
Cisplatin;
Tumor invasion;
Migration
- From:
The Journal of Clinical Anesthesiology
2014;(12):1227-1230
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of morphine combined with cisplatin on inva-sionon and migration of human lung adenocarcinoma cell A549.Methods Human lung adenocarcino-ma A549 cells were inoculated on cultured for 24 h,then were randomly divided into 5 groups:control group (group CON),cisplatin group (group CIS),morphine 0.3 μg/ml+ cisplatin group (group MT1),morphine 3 μg/ml+ cisplatin group (group MT2),morphine 30 μg/ml+ cisplatin group (group MT3).Cisplatin concentration was 4μg/ml.Each group was medicated immediately after 48 h incubation,invasion detection cells by Transwell assay,cell scratch assay cell migration ability, Western-blot detection of matrix metalloproteinase-2 (MMP-2),the expression of MMP-9,Ezrin protein and Fascin protein.Results Compared with group CON,group CIS and morphine combined with cisplatin group reduced tumor cell invasion and migration ability,group MT3 and CIS down-reg-ulated MMP-2,MMP-9,Ezrin and Fascin expression (P<0.05).Compared with group CIS,com-bined with cisplatin group enhanced tumor cell invasion and migration ability group MT3,up-regula-ted MMP-2,MMP-9,Ezrin and Fascin expression (P<0.05).Conclusion Morphine may dose de-pendently reduce cisplatin on invasion and migration of human lung adenocarcinoma cell line A549, and up-regulation of MMP-2,MMP-9,Ezrin,Fascin expression is one of its possible mechanisms.
