Expression and diagnostic value of HBHA truncated protein
10.3969/j.issn.1673-4130.2014.24.029
- VernacularTitle:HBHA截短体蛋白的表达纯化及其诊断活性研究
- Author:
Shan ZHOU
;
Xiuli XU
;
Yueyun MA
;
Jiayun LIU
;
Xiaodong CHENG
;
Qian ZHANG
;
Xiaoke HAO
- Publication Type:Journal Article
- Keywords:
TB;
HBHA;
HBHAΔC;
HBHAΔN
- From:
International Journal of Laboratory Medicine
2014;(24):3366-3368
- CountryChina
- Language:Chinese
-
Abstract:
Objective To express recombinant HBHAΔC and HBHAΔN protein,and compare the HBHA series protein activity with each other.It will be provide a experimental basis for the research on clinical diagnostic of HBHA.Methods The HBHAΔC and HBHAΔN gene fragments were cloned and expressed by transforming E.coli BL-21.Test the protein heparin binding ability by CL-6B column.And then added protein to the BCG 7H9 culture medium,to observe the induced BCG aggregation.Results nHB-HA,rHBHA and HBHAΔN protein have heparin binding ability.Meanwhile nHBHA,rHBHA and HBHA Δ C protein have in-duced BCG aggregation effect.Conclusion The HBHAΔC and HBHAΔN protein were successfully obtained.It was proved that the HBHA C-terminal could be combined with heparin and the N-terminal involved could induce the aggregation of BCG.This results provide a basis for further study on molecular mechanism of TB infection and clinical application.
