Identification of Grain Sprouts Herbs and Their Adulterants Using ITS2 Barcode

VernacularTitle:基于ITS2序列鉴定谷物芽类药材及其混伪品＊
Author: Chao XIONG ; Hong ZHOU ; Haibo HE ; Zhigang HU ; Hegang LIU
Publication Type:Journal Article
Keywords: Grain sprouts herbs; ITS2; identification; adulterants
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(11):2343-2348
CountryChina
Language:Chinese
Abstract: Some grain sprouts herbs recorded in Chinese Pharmacopoeia 2010, including Oryzae Fructus Germinatus, Setariae Fructus Germinatus and Hordei Fructus Germinatus, together with their adulterants, were identified by ITS2 sequences. The genomic DNA were extracted, their ITS2 sequences were amplified, and purified PCR products were
sequenced. Sequences were assembled using the CodonCode Aligner. The genetic distances, variable sites and the neighbor-joining (NJ) phylogenetic tree were computed by MEGA 6.0 in accordance with the Kimura 2-Parameter (K2P) model. The results showed that their intraspecific genetic distances were all much lower than the interspecific ones of themselves and their adulterants. The NJ tree based on ITS2 sequence indicated that Oryza sativa, Setariaitalica, and Hordeum vulgar formed one monophyletic clade respectively, in addition any one of the grain sprouts herbs and its adulterants could be distinguished clearly. Therefore, ITS2 sequence is suitable to be as a barcode to identify the grain sprouts herbs and their adulterants.