Identification of “Gonglao Leaf” and Its Sibling Adulterants Based on ITS2 Sequence
10.11842/wst.2014.11.013
- VernacularTitle:ITS2序列鉴定“功劳叶”药材及其近缘混伪品＊
- Author:
Ye XIA
;
Hong ZHOU
;
Li XIANG
;
Xiuqiao ZHANG
;
Zhigang HU
- Publication Type:Journal Article
- Keywords:
Ilex cornuta;
gonglao leaf;
ITS2 sequence;
molecular identification;
sibling adulterants
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(11):2361-2365
- CountryChina
- Language:Chinese
-
Abstract:
Objective: The Phenomenon of different species with the same name has created potential threats to cinical safety of medication. Because of that, two kinds of “gonglao leaf” and their sibling adulterants have been identified by molecular methods in this study, to secure its safety in medication. Methods: Ilexcornuta, Mahoniabealei, Mahoniafortuneiand their sibling adulterants total of 9 species 45 samples were collected in this experiment,Total genomic DNA was extracted from them by the method of improved CTAB, ITS2 sequences were amplified, and the PCR products were sequenced. Sequence assembly and consensus sequence generation were performed by the CodonCode Aligner V 4.2.4.. The genetic distances were computed by MEGA 6.0 in accordance with the Kimura 2-Parameter (K2P) model and the phylogenetic tree constructed by the neighbor-joining (NJ) method. Results: The analysis results of the genetic distances, variable sites and the NJ phylogenetic tree showed that Ilexcornuta, Mahoniabealei, Mahoniafortunei were seperated from their sibling adulterants obviously. Conclusion: ITS2 sequence was able to identify two kinds of “gonglao leaf” and their sibling adulterants which can provide a basis for clinical accurate medication.
