Proteome Analysis of Ampelopsis Megalophylla by 2-DE
10.11842/wst.2014.11.016
- VernacularTitle:大叶蛇葡萄蛋白质组双向电泳分析＊
- Author:
Jing HUANG
;
Chun GUI
;
Ye XIA
- Publication Type:Journal Article
- Keywords:
A mpelopsis megalophylla;
Proteomics;
Protein extraction;
2-DE
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(11):2380-2384
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate active ingredients from A mp e lop s is me galop hylla of protein expression by using two-dimensional gel electrophoresis (2-DE). Methods: isn this study, proteinsfrom the tender leaves of A mpelopsis megalophylla were precipitated by Tris-phenol extraction and ammonium acetate methanol precipitation. Then using 2-DE technology to isolate protein.Finally, 2-DE was analyzed by the scanner, and got protein characteristic maps. Result:According to the differences in ratio of gray value, the seven differential protein points were chosen to identify the mass spectrum. At present, two proteins were identified, namely the hypothetical protein and the unnamed protein product. Conclusion: the application of 2-DE technology for A mpelopsis megalophyllathe active ingredient of protein expression at the level of molecular research laid a good foundation.
