Optimization of Extraction Technology for Total Saponins in Dipsaci Radix by Central Composite Design-Response Surface Methodology

VernacularTitle:星点设计-响应面法优化续断总皂苷提取工艺＊
Author: Qing GE ; Xixi FAN ; Cuixiang PENG ; Hanlin XU
Publication Type:Journal Article
Keywords: Dipsaci Radix; the total saponins; response surface methodology; extraction process
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(11):2395-2399
CountryChina
Language:Chinese
Abstract: To optimize the extraction technology of the total saponins in Dipsaci Radix, the extraction efficiency of total saponins was investigated with respecting to three variables including time, ethanol concentration and liquid-to-solid ratio. On the basis of a series of one-factor-at-a-time experiments, a polynomial regression model equation was fitted by the combined use of central composite experimental design and regression analysis. By analyzing the regression model using response surface analysis, the optimum extraction conditions of total saponins from Dipsaci Radix were identified as follows: 7 times of 55% alcohol, refluxing 3 times, 2.8 hours every time, and the extraction efficiency of total saponins was up to 0.201 1 g·g-1 and the asperosaponin Ⅵ was up to 0.015 4 g·g-1 under the optimized conditions. Confirmatory experiment indicated the good prediction ability of the established model and provided the basis for further development and utilization of Dipsaci Radix.