Model Establishment and Coagulation Changes in Rats with Acute Liver Failure
10.11842/wst.2014.11.021
- VernacularTitle:大鼠急性肝功能衰竭模型的建立及凝血功能的变化＊
- Author:
Jianxin DIAO
;
Wenxiao MA
;
Yawei LIU
;
Heyu HUA
;
Yungao YANG
- Publication Type:Journal Article
- Keywords:
D-galactosamine;
lipopolysaccharide;
acute liver failure;
ALT;
AST
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(11):2406-2410
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to observe effects of different doses of D-galactosamine (D-GalN) plus lipopoly-saccharide (LPS) and blood coagulation changes among rat model of acute liver failure, in order to establish an ideal model of acute liver failure in rats. SD rats were randomly divided into the control group, D-GalN high, medium and low dose groups, with 10 rats in each group. Except the normal group, rats in other groups were injected with D-GalN plus LPS at different doses to induce acute liver failure. The mortality of rats was observed. The liver function and blood coagulation were detected from rat serum at 0 h, 12 h, 24 h, 48 h, and 72 h. HE stain was used in the observa-tion of changes on liver pathological changes. The results showed that the mortality of D-GalN high, medium and low dose groups within 72 h were 60%, 30%, 10%, respectively. There were significant differences on the serum content level of ALT, AST, TBIL, PT, INR, FIB from different dose groups at different time points and the normal group (P<0.05). However, the comparison among D-GalN high, medium and low dose groups showed no statistical difference on ALT and AST; while there were statistical differences on TBIL, PT, INR and FIB (P < 0.05). It was concluded that coagulation index was more stable in the liver failure model. Through observation on the liver function, blood coagulation and pathological morphology, the model of acute liver failure induced with medium dose of D-GalN plus LPS in SD rats at 48 h was more similar to the clinical symptom of acute liver failure. Therefore, the medium dose was the ideal model inducing dose.
