Bioinformatics Analysis on Proteomics of Rat Bone Marrow Mesenchymal Stem Cells Intervened by Active Principle Region of Yang-Xin Tong-Mai Formula
10.11842/wst.2014.11.031
- VernacularTitle:养心通脉有效部位方对大鼠骨髓间充质干细胞作用蛋白组学的生物信息学分析＊
- Author:
Jinghui ZHENG
;
Zhaokai YUAN
;
Longjian HUANG
;
Xinzheng WU
;
Weixiong JIAN
;
Xianping HUANG
;
Jiyong LIU
;
Gang ZENG
;
Tiehua WANG
;
Liang TANG
- Publication Type:Journal Article
- Keywords:
Active principle region of Yang-Xin Tong-Mai Formula;
bone marrow mesenchymal stem cells;
proteomics;
bioinformatics
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(11):2460-2469
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to analyze the bioinformatics of proteomics of rat bone marrow mesenchymal stem cells (MSCs) intervened by active principle region of Yang-Xin Tong-Mai Formula (apr-YTF). The latest versions of bioinformatics tools including DAVID (http://david.abcc.ncifcrf.gov/) and GO (http://www.geneontology.org/) were combined to assign a precise function to rat bone marrow MSCs intervened by apr-YTF. KEGG and VISANT were assigned with a precise function to rat bone marrow MSCs intervened by apr-YTF. The results showed that a total of 102 biological processes were mainly involved, with 35 cellular components and 6 molecular functions. These proteins interacted in 3 signal transduction pathways. It was concluded that the following proteins and signal transduction pathways played an important role in the process of apr-YTF inducing BMSCs differentiation into cardiomyocytes. Presenilin-1 and Presenilin-2 were in the Notch signaling pathway. And syntaxin-4 protein was in soluble N-ethylmaleimide sensitive fusion protein attachment protein (SNARE). The apr-YTF played a role on MSCs from multiple sites, with multiple links through different biological processes. The bioinformatics of proteomics can predict action mechanism of traditional Chinese medicine (TCM) from the holism concept. The validation in combination with molecularbiology was a good way for TCM modernization.
