Extracts of Celastrus Orbiculatus Enhance the Invasion Function in Maspin Overexpression Human Gastric Carcinoma MGC803 Cells
10.11842/wst.2014.11.032
- VernacularTitle:南蛇藤提取物增强抑癌基因Maspin抑制人胃癌细胞株MGC803侵袭能力的研究＊
- Author:
Yayun QIAN
;
Ling CAO
;
Yanqing LIU
;
Xin JIANG
;
Lanfang JI
;
Lin YANG
;
Yaodong ZHU
;
Jue CHEN
;
Hao GU
;
Feng JIN
;
Yunfei BAI
- Publication Type:Journal Article
- Keywords:
Celastrus orbiculatus;
maspin;
gastric carcinoma;
invasion
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(11):2470-2474
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate whether the extracts of Celastrus orbiculatus enhanced the invasion function of maspin tumor inhibitor gene through the construction of maspin overexpression human gastric carcinoma MGC803 cell line. Maspin was cloned into plasmid GV208-EGFP eukaryotic expression vector. And then, the recombinant plasmid GV208-maspin-EGFP was transfected into human gastric carcinoma MGC803 cells. After the maspin overexpression MGC803 cell were treated with Celastrus orbiculatus extracts in different concentrations (10, 20, 40 μg·mL-1), the invasion effects were detected by Transwell chamber assay. The results showed that after the successful construction of maspin overexpression cell line, the number of cells invading through Matrigel was obviously decreased in the Transwell chamber assay. It also showed drug concentration dependency. It was concluded that maspin gene can inhibit invasion of gastric carcinoma MGC803 cells. Simultaneously, the extracts of Celastrus orbiculatus can enhance the function of maspin gene.
