Relationship between blood circulation miRˉ21 expression and clinicopathological features of gastric cancer before and after gastric surgery
10.3969/j.issn.1673-4130.2014.24.008
- VernacularTitle:胃癌手术前后血循环miR-21表达与胃癌临床病理特征的关系研究
- Author:
Rongmin GU
;
Min ZHU
;
Xu WEN
;
Senqing CHEN
;
Jintian LI
;
Guojian MA
- Publication Type:Journal Article
- Keywords:
peripheral blood;
gastric cancer;
fluorescence quantitative PCR
- From:
International Journal of Laboratory Medicine
2014;(24):3316-3318
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effect of peripheral blood miR-21 expression on the clinicopathological features of gastric cancer before and after gastric surgery.Methods The peripheral blood was collected on the operative day and postoperative 7 d in 42 patients with primary gastric cancer surgery.The real-tine quantitative PCR was adopted to detect the relative expression of miR-21 in plasma with U6 as an internal reference gene.Results The plasma miR-21 expression level after gastric cancer operation was significantly down-regulated compared with before operation(P <0.01),the overall relative expression level was dropped by an average of 18.2 times;and it was found that the postoperative miR-21 expression in the patients with the tumor family history was up-regulated,the postoperative plasma miR-21 expression in the patients without the tumor family history was decreased by 22 times compared with before operation(P <0.05);the experimental results also fund that as the patient′s differentiation degree was changed from high to low,the lymph node metastasis rate was gradually increased,while postoperative average miR-21 expression was changed from low to high,showing the relative expression times of miR-21 were distributed in the four differentiated zones of high,middle,middle-low and low,which showed the correlation with the distribution of the lymph node metastasis rate in these four differentiation zones.Conclusion The peripheral blood MiR-21 detection has the clinical significance to early auxiliary diagnosis and the judgement of postoperative malignant degree.
