Establishment of TaqManˉBHQ probe realˉtime fluorescence RTˉPCR for quantitative detection of Soul virus
10.3969/j.issn.1673-4130.2014.24.014
- VernacularTitle:汉城病毒的TaqMan-BHO探针实时荧光定量检测方法的建立
- Author:
Chuncheng LIU
;
Pengfei YANG
;
Qingli YAN
;
Yujun SHI
- Publication Type:Journal Article
- Keywords:
TaqMan-BHQ probe;
real-time fluorescence RT-PCR;
soul virus;
quantitative detection
- From:
International Journal of Laboratory Medicine
2014;(24):3332-3333
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a rapid method of real-time fluorescence RT-PCR to quantify Soul virus.Methods The pro-fessional software was adopted to design the primer and the TaqMan-BHQ probe.With artificially synthesized L gene segment as the template of Soul virus,the real-time RT-PCR for detecting Soul virus was researched.Results The Ct value of templates had a good linear relationship with the log value of the template diluted concentration.The standard curve was Y =-3.607X +41.84, r2 =0.998,the PCR amplification efficiency was 108.1%,its lowest detection limit was 53.2 copies/μL.Conclusion Applying the real-time fluorescence RT-PCR by the TaqMan-BHQ probe for detecting nucleic acid of Seoul virus has the characteristics of short time-consuming and high sensitivity.
