Effect of ethyl pyruvate on E-cadherin of airway epithelium in a TDI-induced mouse asthma
10.3969/j.issn.1006-5725.2014.22.005
- VernacularTitle:丙酮酸乙酯对TDI诱导的哮喘小鼠气道上皮细胞黏附连接蛋白E-cadherin的影响
- Author:
Junjie LIANG
;
Haixiong TANG
;
Haijin ZHAO
;
Jiafu SONG
;
Lihong YAO
;
Hangming DONG
;
Shaoxi CAI
- Publication Type:Journal Article
- Keywords:
Ethyl pyruvate;
TDI-induced asthma;
E-cadherin
- From:
The Journal of Practical Medicine
2014;(22):3555-3558
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of ethyl pyruvate (EP) on E-cadherin of airway epithelium and airway inflammation in a TDI-induced mouse asthma model. Methods 30 male BALB/c mice were randomly divided into control group , asthma group and EP group. On day 1 and 8 , mice in asthma group and EP group were treated with 0.3%TDI on the dorsum of both ears for sensitization. And on day 15 , 18 and 21 the mice underwent an aerosol inhalation of 3% TDI, and saline (100 mg/kg) was injected intraperitoneally 1 hour before inhalation. The control group underwent acetone and olive oil (AOO) sensitization on day 1 and 8, AOO challenge on day 15, 18 and 21. Saline (100 mg/kg) was injected intraperitoneally 1 hour before challenge. One hour before each challenge, mice were given EP (100mg/kg) or vehicle via intraperitoneal injection. On day 22, airway reactivity, IL-4 , IFN-γand IgE in the serum were detected , immunohistochemistry and WB were used to assess E-cadherin levels. Results Airway reactivity, IL-4, IFN-γin and IgE in the serum in asthma group are significantly higher than that in control group (P<0.05). Treatment with EP dramatically decreased airway hyperresponsiveness in TDI-challenged mice, as well as IL-4, IFN-γ and IgE (P < 0.05). E-cadherin in control group was distributed evenly at the connection of epithelial cells. E-cadherinin distribution was chaotic and its expression was decreased in asthma group. EP intervention can ameliorate the damage of E-cadherinin. Conclusions EP can ameliorate the destruction of E-cadherin in airway epithilum by TDI.
