The mechanism of apoptosis regulation of transcription factor E2F1 in small cell lung cancer cell
10.3969/j.issn.1006-5725.2014.22.010
- VernacularTitle:转录因子E2F1调控小细胞肺癌凋亡机制的研究
- Author:
Shan SU
;
Huiyi HUANG
;
Jianjun ZHOU
;
Wenchang CEN
;
Xianlan ZHANG
- Publication Type:Journal Article
- Keywords:
Small Cell Carcinoma;
Apoptosis;
E2F1 Transcription Factor
- From:
The Journal of Practical Medicine
2014;(22):3573-3575
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of transcription factor E2F1 on the apoptosis of small cell cancer line H446. Methods Plamid vector- mediated E2F1 small hairpin RNA (shRNA) was used to silence E2F1 in H446 cell. RT-PCR and western-blot assay were used to detect the expressions of E2F1 and Bcl-2. The apoptosis rate in H446 cell line was detected by flow cytometry assay. Result E2F1 protein was suppressed in shRNA1-modified H446 cell. Sgnificant difference of the apoptosis was shown between E2F1 shRNA1 group and the other two groups. Additionaly, the expression of Bcl-2 protein increased in E2F1 shRNA1-modified cell line. Conclusions E2F1 is highly expressed in H446 small cell lung cancer cell line. E2F1 promotes apoptosis of H446 through upregulating Bcl-2 expression.
