Screening and Fingerprint Analysis of Active Site to Reduce Blood Lipid from Xin-Mai Capsule
10.11842/wst.2014.11.035
- VernacularTitle:欣脉胶囊降血脂活性部位的筛选及其指纹图谱归属分析＊
- Author:
Yinhua DING
;
Wei SHI
;
Yongcheng SUN
;
Xinyong LYU
;
Jun ZHOU
;
Zhenzhong WANG
;
Yuan BI
;
Wei XIAO
- Publication Type:Journal Article
- Keywords:
Xin-Mai capsule;
reducing blood lipid;
active site;
fingerprint;
correlativity;
HPLC
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(11):2487-2492
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to screen main active site to reduce blood lipid from Xin-Mai (XM) capsule and establish HPLC fingerprint of the site, in order to study the correlativity between active site and relevant fractions of its herbs. Solvent extraction was used to separate XM capsule into different polar fractions. Intraperitoneal injection of 75% egg-yolk emulsion was used to establish mice hyperlipidemia models. And the active site was screened. Chromatographic fingerprints of the site and relevant fractions of its herbs were configured by HPLC analysis. The
retention time of peaks was utilized as index to evaluate the correlativity. The results showed that lipid-lowering effect of ethyl acetate extract and garlic essential oil was significant (P<0.01). Fingerprint of the active site in XM capsule was established with 28 fingerprint peaks and the assignment results of 27 peaks were indicated. It was concluded that the active sites to reduce blood lipid of XM capsule were ethyl acetate extract and garlic essential oil. The established fingerprint method can effectively determine the correlativity between the active site and its relevant fractions, which contributed to pharmacodynamic material foundation and quality standard.
