Optimization of Bio-Solid Bidirectional Fermentation Conditions of Trametes Robiniophia Murr . for Rhubarb

VernacularTitle:槐耳大黄双向发酵体系研究＊
Author: Li ZHOU ; Cencong QI ; Pengfei GAO ; Rongrong ZHOU ; Wenyi ZHANG ; Xinyuan SHI
Publication Type:Journal Article
Keywords: Trametes robiniophila Murr.; rhubarb; bidirectional fermentation; process optimization
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(11):2500-2505
CountryChina
Language:Chinese
Abstract: This study was aimed to optimize bio-solid bidirectional fermentation conditions of Trametes robiniophia Murr. for rhubarb. Sulphuric acid-phenol colorimetry, magnesium-methanol colorimetry, and HPLC were used in the
content determination of polysaccharide, total anthraquinone, and 4 free anthraquinones. The drying rate and con-sumption rate were combined as indicators for the optimization of technical parameters such as medicinal dosage, temperature and amount of water. The results showed that when using 500 mL conical flask, the best fermentation conditions were medicinal dosage of 10 g, the temperature of 34℃, adding water of 120%. It was concluded that bidirectional fermentation of rhubarb increased the content of free anthraquinones. Among them, the content of chrysophanol with anti-oxidation effect increased significantly. The decreasing of combined anthraquinone can relieve the severe laxative effect of rhubarb.