Effects of Radix Hedysari Flavonoids on Expressions of MMP-2 and TIMP-1 Protein in Rat Pulmonary Fibrosis Model
10.3969/j.issn.1005-5304.2015.01.014
- VernacularTitle:红芪黄酮对肺纤维化模型大鼠基质金属蛋白酶-2及其抑制剂-1蛋白表达的影响
- Author:
Yun SU
;
Yi ZHANG
;
Juan LI
;
Guangwei LI
;
Xueyan LI
;
Xingyao LIN
;
Jintian LI
;
Ming JING
- Publication Type:Journal Article
- Keywords:
pulmonary fibrosis;
radix hedysari flavonoids;
MMP-2;
TIMP-1;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(1):47-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss radix hedysari flavonoids mechanism of preventing pulmonary fibrosis.Methods Totally 216 SPF Wistar rats were randomized into normal group, model group, prednisone group, radix hedysari flavonoids high, medium, and low dose groups, and then pulmonary fibrosis model was established by intratracheal dripping of bleomycin. From the second day after modeling, every treatment group received gavage with the corresponding dose of medicine at the planned time for 7, 14, and 28 continuous days. Expressions of MMP-2 and TIMP-1 protein were detected by immunohistochemical method.Results When radix hedysari flavonoids high dose group was at the 7th, 14th, 28th day, and medium dose group was at the 14th day, MMP-2 protein expression was lower than model group, similar to prednisone group. When radix hedysari flavonoids high dose group was at the 14th, 28th day and medium dose group was at 14th day, TIMP-1 protein expression was lower than model group.Conclusion Radix hedysari flavonoids can adjust the expressions of MMP-2 and TIMP-1 protein at different phases, and tend to make the balance of MMPs/TIMPs, which may be an effective mechanism for the inhibition of fibrosis process.
