Effects of Thin Recipe ofBuyang Huanwu Decoction on Expressions of GSH, GSH-Px andγ-GCS in the Brain of Focal Cerebral Ischemia Rats
10.3969/j.issn.1005-5304.2015.01.017
- VernacularTitle:补阳还五汤精简方对局灶性脑缺血大鼠脑谷胱甘肽抗氧化系统的影响
- Author:
Yan SHE
;
Jian YI
;
Le SHAO
;
Xiangyi XIA
;
Fang LIU
;
Zhoujin TAN
;
Guangxian CAI
;
Yuhong WANG
- Publication Type:Journal Article
- Keywords:
thin recipe ofBuyang Huanwu Decoction;
focal cerebral ischemia;
oxidative stress;
GSH;
GSH-Px;
γ-GCS;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(1):58-61
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of thin recipe ofBuyang Huanwu Decoction on expressions of glutathione (GSH) antioxidant system including GSH, GSH-Px andγ-GCS in the brain of focal cerebral ischemia rats;To study its mechanism of antioxidant effect.Methods Totally 120 SD rats were randomly divided into four groups according to random number table method:sham-operation group, model group,Buyang Huanwu Decoction group and thin recipe ofBuyang Huanwu Decoction group. Except for the sham-operation group, the rest groups established focal cerebral ischemia rat model by middle cerebral arterial occlusion. The treatment groups were given corresponding medicine by gavage, while the sham-operation group and model group were given the same amount of normal saline 2 h after modeling. Each group was detected after cerebral ischemia for 1 day, 3 days, and 7 days respectively. The content of GSH and the activity of GSH-Px were detected. At the same time,γ-GCS mRNA and protein levels were determined by using real time-PCR and Western blot.Results The content of GSH and the activity of GSH-Px at each time point decreased in the model group (P<0.05), compared with the corresponding sham-operation group. But the expression ofγ-GCS mRNA and protein increased, with statistical significance on day 1 (P<0.05). Compared with model group, content of GSH and GSH-Px activity levels increased differently in each treatment group, whileγ-GCS mRNA and protein expression raised, with statistical significance on day 3 (P<0.05).Conclusion The thin recipe ofBuyang Huanwu Decoction plays antioxidant effect by regulating the expression of glutathione antioxidant system GSH, GSH-Px andγ-GCS after cerebral ischemia, which may be one of its therapeutic mechanisms for cerebral ischemia.
