Effects of Chinese FormulaXuetongling on MDA-MB-231 Human Breast Cancer Cell Invasion and MMP-9 Protein Expression
10.3969/j.issn.1005-5304.2015.01.019
- VernacularTitle:不同浓度血通灵对人乳腺癌细胞侵袭作用及基质金属蛋白酶-9蛋白表达的影响
- Author:
Lan YAO
;
Hongmei MA
;
Bingjie DU
;
Jaiheon LEE
;
Zheng CUI
- Publication Type:Journal Article
- Keywords:
Xuetongling;
MDA-MB-231;
invasion assay;
MMP-9
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(1):67-69
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the action mechanism of Chinese FormulaXuetongling on MDA-MB-231 human breast cancer cell invasion.Methods Transwell Invasion assay was applied to investigate the inhibitory effects on cancer cell invasion ofXuetongling extract in different concentrations;MMP-9 secretion activity was detected by zymography assay after the treatment of XTL extract in MDA-MB-231;Western blot was used to detect the effect of XTL extract on MMP-9 protein expression in MDA-MB-231.ResultsXuetongling extract in different concentrations significantly suppressed the cell invasion, MMP-9 secretion and MMP-9 protein expression in dose dependent manner.Conclusion The inhibitory effect ofXuetongling on MDA-MB-231 cell invasion may be due to the down-regulation of both MMP-9 secretion and MMP-9 protein expression.
