A Study on Quality Standard Improvement forZushima Plaster

VernacularTitle:祖师麻膏药质量标准的提高研究
Author: Li LIN ; Bin FAN ; Xiaofeng SHI ; Zhuhong MAO
Publication Type:Journal Article
Keywords: Zushima Plaster; quality control; daphnetin; 7-hydroxycoum; TLC; HPLC
From: Chinese Journal of Information on Traditional Chinese Medicine 2015;(1):83-86
CountryChina
Language:Chinese
Abstract: Objective To improve the quality standard ofZushima Plaster.Methods TLC was used to do qualitative identification of daphnetin and 7-hydroxycoumarin inZushima Plaster. The contents of daphnetin and 7-hydroxycoumarin inZushima Plaster were determined by HPLC. The chromatographic column was Agilent Eclipse Plus C18 (4.6 mm×250 mm, 5μm), and the mobile phase consisted of acetonitrile-0.1% phosphoric asid (15∶85, V/V) at a flow rate of 1.0 mL/min. The column temperature was maintained at 30℃, and the detection wavelength was set at 327 nm. Results Characteristic spots of daphnetin and 7-hydroxycoumarin could be detected by TLC from the samples, and the spots were clear and specific without interference from negative samples. The calibration curve of daphnetin and 7-hydroxycoumarin were linear in the ranges of 0.180-1.800μg and 0.100-1.00μg (r=0.999 1,r=0.999 2), and the average recovery rates were 98.99% and 101.48%, RSDwere1.43% and 1.32%, respectively (n=9).Conclusion The established method appears easy to use, accurate and specific, and therefore can be used for the quality control ofZushima Plaster.