Effect of 20 and 22 amino acid on superantigen activity of staphylococcal enterotoxin C2
10.3969/j.issn.1000-484X.2014.12.003
- VernacularTitle:肠毒素C2第20和22位氨基酸对其超抗原活性的影响
- Author:
Yanli LIU
;
Rongcheng NIU
;
Mingkai XU
;
Xu LI
;
Zhencheng SU
;
Huiwen ZHANG
- Publication Type:Journal Article
- Keywords:
Staphylococcal enterotoxin C 2;
Superantigen;
T cell;
Cytokine
- From:
Chinese Journal of Immunology
2014;(12):1596-1600
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the improved superantigen activity of SEC2(T20L/G22E) compared with recombinant staphylococcal enterotoxins C 2 ( rSEC2 ).Methods: The proliferation of spleen lymphocytes and T-cell subpopulations induced by rSEC2 and SEC2(T20L/G22E) were examined by WST-1 and flow cytometry separately,and the gene expression of cytokines and Vβspecificities were quantified by real-time PCR.Results: WST-1 and Flow cytometry assays showed that the superantigen activity of SEC2(T20L/G22E) was improved due to enhanced T-cell stimulating potency,resulting in massive activation of T-cells,particularly CD4+and CD8+T-cells.Quantitative real-time PCR assay showed that despite similar Vβspecificities induced by rSEC 2 and SEC2 (T20L/G22E),the quantities of activated T-cells bearing specific Vβwere different,and SEC2(T20L/G22E) could stimulate more gene expression of associated cytokines simultaneously.Conclusion: The results strongly suggested that the increased SEC 2 ( T20L/G22 E)-TCR-binding affinity contributed to more T-cells activation and cytokine release ,which elicit powerful immune activition.
