Mesenchymal stem cell-conditioned medium could ameliorated triptolide induced damage in KGN cells
10.3969/j.issn.1000-484X.2014.12.012
- VernacularTitle:MSCs上清可减轻雷公藤甲素对KGN细胞的损伤作用
- Author:
Xue ZHOU
;
Guangfeng ZHAO
;
Shiwen CHEN
;
Yayi HOU
- Publication Type:Journal Article
- Keywords:
Mesenchymal stem cells (MSCs);
Ovarian granulosa cells;
Triptolide;
CDKN1A
- From:
Chinese Journal of Immunology
2014;(12):1641-1646
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the ameliorative effect and machanism of MSCs conditioned medium on the ovarian granulosa cells damage induced by triptolide.Methods: Cell Counting Kit 8 assay was used to examine the cell vitality of KGNs with the treatment of triptolide.The mixed enzyme digestion method were used for the isolation of human umbilical cord mesenchymal stem cells (MSCs),and flow cytometry was used for the subsequent immunotype identification.MSCs conditioned medium was collected ,and Cell Counting Kit 8 assay and PI staining was used to analyse the effect of MSCs conditioned medium on the cell vitality and cell cycle distri -bution of triptolide-damaged KGN.Real-time PCR method was used to examine the expression of cell cycle related gene CDKN1A.Results:Triptolide can inhibit KGN cell growth with the inhibition of cell vitality and cell cycle of KGN.MSCs conditioned medium did not influence the proliferation and cell cycle of normal KGN , but improved the triptolide-induced vitality inhibition and extent of S-phase arrest, and inhibit the abnormal up-regulation of CDKN1A in KGN.Conclusion: MSCs conditioned medium ameliorated the KGN cell damage induced by triptolide.
