"Transplant human PBMC to establish a ""human-mouse""xenogeneic graft-versus-host disease model"
- VernacularTitle:输注人PBMC建立“人-鼠”异种移植物抗宿主病模型
- Author:
Jun CHEN
;
Wei ZHANG
;
Xiufang WENG
;
Shengjun LU
;
Xiongwen WU
;
Zhihui LIANG
- Publication Type:Journal Article
- Keywords:
PBMC;
Organ transplantation;
Xenotransplantation;
Graft versus host disease
- From:
Chinese Journal of Immunology
2014;(12):1658-1661
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the suitable mouse strains,and establish stable human-miceX-GVHD model.Methods:This study selected the Nude Mice and NOD/SCID, and gave them sublethal dose of γ-ray irradiation whole body , and then intraperitoneal transplanted human peripheral blood mononuclear cells ( PBMC) to establish xenogeneic acute graft-versus-host disease model.By detection of human T cells in the mice′s tail venous blood,tissues,organs of the infiltration and other indicators (By flow cy-tometry and immunohistochemistry ) ,we compared the human immune cell infiltration rates in the two mouse model and recorded the survival time.Finally,we determined the appropriate strains of mice to establish X-GVHD.Optimization means were transfer ways and the appropriate amount of human PBMC ,and the best time to observe the changes of T cell phenotype and function.Results:The NOD/SCID mouse was more suitable for inducing human-mouseX-GVHD model,and there were no significant differences between intrap-eritoneal injection and intravenous injection.Transfer human PBMC more than 5 ×107 can establish human-mouseX-GVHD model.Using the optimized experimental conditions to establish the human-mouseX-GVHD model,we found the 7-11 days was the best time to observe the changes of T cell phenotype and function ,and the average survival time was(14.16±1.77)days.Conclusion:Human-miceX-GVHD model can be successfully established by intraperitoneal injection of 5×107 human PBMC into NOD/SCID, and the best time to observe the changes of T cell phenotype and function is between 7-11 days.
