Effects ofCangfu Congxian Decoction on mRNA Expression of IGF-1R in Human Mural Granulosa Cells of PCOS Patients
10.3969/j.issn.1005-5304.2015.01.009
- VernacularTitle:苍附苁仙汤对多囊卵巢综合征患者卵巢颗粒细胞胰岛素样生长因子-1受体基因表达的影响
- Author:
Ying LIANG
;
Huilan DU
;
Yuxia MU
- Publication Type:Journal Article
- Keywords:
Cangfu CongxianDecoction;
polycystic ovary syndrome;
mural granulosa cell;
IGF-1R;
high quality embryo
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(1):29-32
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects ofCangfu Congxian Decoction on the mRNA expression of IGF-1R in human mural granulosa cells;To explore the action mechanism for the treatment of polycystic ovary syndrome (PCOS) patients.Methods Totally 48 patients undergoing IVF-ET were randomly divided into treatment group and control group by random number table, 24 cases in each group. Patients in the control group accepted GnRHa/FSH/hCG stimulation, while the treatment group tookCangfu Congxian Decoction orally on the basic treatment of the control group. Serum E2, LH, and P concentration on HCG injection day, number of ovum, high quality embryo rate and clinical pregnancy rate were evaluated. The mRNA expression of IGF-1R on mural granulosa cells in the mature follicle was detected by real-time PCR.Results Compared with the control group, there was no significant difference in serum E2, LH, and P concentration on HCG injection day of treatment group (P>0.05), while the treatment group had higher high quality embryo rate than that of the control group (P<0.01). There was no significant difference in the number of ovum and pregnancy rate between the two groups (P>0.05). The mRNA expression of IGF-1R on granulosa cells in treatment group was significantly higher than the control group (P<0.01).Conclusion The mechanism ofCangfu Congxian Decoction for treating PCOS is up-regulating the mRNA expression of IGF-1R in mural granulosa cells and increasing high quality embryo rate.
