Effects ofTanyu Tongzhi Formula Drug-Containing Serum on Proliferation and Migration of Human Umbilical Vein Endothelial Cells

VernacularTitle:痰瘀同治方药物血清对人脐静脉内皮细胞增殖和迁移的影响
Author: Sheng ZHU ; Jianxun LIU
Publication Type:Journal Article
Keywords: Tanyu Tongzhi Formula; human umbilical vein endothelial cells; cell proliferation; cell migration
From: Chinese Journal of Information on Traditional Chinese Medicine 2015;(1):50-52
CountryChina
Language:Chinese
Abstract: Objective To observe the effects ofTanyu Tongzhi Formula on proliferation and migration of human umbilical vein endothelial cells (HUVECs);To explore its activity of pro-angiogenesis.Methods HUVECs were cultured in vitro. Rats were treated with gavage containing different doses ofTanyu Tongzhi Formula (78.0, 39.0, 19.5 g/kg) to prepare serum with different doses of medicine. The activity of HUVECs was measured by MTT method. The effects on migration of HUVECs were inspected by using scratch wound assay.Results Compared with the normal control group, serum containing high-dose and medium-doseTanyu Tongzhi Formula could significantly promote the proliferation of the HUVECs (P<0.01). The rates were 49.78% and 33.92% respectively. And the cell migration rate was higher in the two groups than in the normal control group (P<0.01). The rates were 11.36% and 11.62% respectively.ConclusionTanyu Tongzhi Formula can promote proliferation and migration in HUVEC, which may be active to pro-angiogenesis.