Study of killing effect on liver cancer cells by transfected dendritic cell vaccine of recombinant plasmid pchIL-18-MAGE in vitro
10.3969/j.issn.1000-484X.2014.12.005
- VernacularTitle:重组质粒 pchIL-18-MAGE 转染树突状细胞疫苗体外杀伤肝癌细胞的研究
- Author:
Binbin DIAO
;
Shouqing LI
;
Yinfu MA
;
Guangmin YANG
- Publication Type:Journal Article
- Keywords:
Vaccine;
Hepatocellular carcinoma;
Dendritic cells;
MAGE-1;
IL-18
- From:
Chinese Journal of Immunology
2014;(12):1606-1610
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe lethal effect on liver cancer cells by the DCs transfected with recombinant plasmid pchIL -18-MAGE.Methods:Cultivate dendritic cells in vitro ,and recombinant plasmid pchIL-18-MAGE was constructed which was transfected into DCs.Expression of IL-18 and MAGE-1 on the transfected DCs were detected by RT-PCR and Western blot.The diversity of DCs phenotypes after transfect were analyzed by flow cytometry.Different killing effects to the target cells by lymphocytes which were stimulated by DC and those by non-transfected DC were detected.IFN-γin the cellular supernatant of the transfected DCs was inspected by ELISA.Results:pchIL-18-MAGE transfected DCs expressed higher level of CD 83,CD1a,CD86,CD80,HLA-DR,and expressed as a mature DC phenotype.pchIL-18-MAGE group′s killing activity was the highest ( P<0.05 ) .Conclusion: The DC vaccine pchlL-18-MAGE can induce better killing effect compared with other experimental groups.
