MicroRNA-378*enhances apoptosis of human mesenchymal stem cells by repressing expression of CTGF
10.3969/j.issn.1000-4718.2014.12.021
- VernacularTitle:MicroRNA-378*通过抑制CTGF促进人骨髓间充质干细胞凋亡
- Author:
Jun DONG
;
Bohang ZENG
;
Ningning LIU
;
Pei MO
;
Longgen XIONG
;
Shiming LIU
;
Jiao LI
- Publication Type:Journal Article
- Keywords:
Human mesenchymal stem cells;
MicroRNA-378*;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2014;(12):2238-2242
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To investigate the effects of microRNA-378*( miR-378*) on the survival and apoptosis of human mesenchymal stem cells ( hMSCs ) .METHODS: The expression of miR-378* was determined by microRNA arrays and quantitative real-time PCR ( qRT-PCR) .H2 O2 was used to induce hMSCs apoptosis.By transfection of miR-378*mimic or inhibitor, we up-regulated or down-regulated miR-378* expression in hMSCs.The effect of miR-378*and connective tissue growth factor ( CTGF) on hMSC survival and apoptosis were detected by MTT, LDH, caspase-3/7 and TUNEL assays.RESULTS:The expression of miR-378*was up-regulated in the old hMSCs compared with the young hMSCs.H2 O2 increased the expression of miR-378*, decreased the expression of CTGF.Up-regulation of miR-378*re-sulted in increasing apoptosis and decreasing survival of hMSCs.Conversely, down-regulation of miR-378*resulted in de-creasing cell apoptosis and increasing survival.The regulation of miR-378*on hMSC apoptosis and survival was attenuated by inhibiting the expression of miR-378* and CTGF together.Direct repression of CTGF expression inhibited the hMSC survival and increased apoptosis.CONCLUSION:miR-378*enhances apoptosis of hMSCs by repressing the expression of CTGF.
