Effects of RNA interference targeting CDC25a gene on proliferation of human liver cancer HepG2 cells
10.3969/j.issn.1000-4718.2014.12.005
- VernacularTitle:RNA干扰技术沉默CDC25a基因对人肝癌细胞HepG2增殖的影响
- Author:
Wei LI
;
Ji CAO
;
Lingli ZHOU
;
Wang LUO
;
Chun YANG
;
Chengpiao LUO
;
Yuan LI
;
Jianjia SU
- Publication Type:Journal Article
- Keywords:
Genes,CDC25a;
RNA interference;
Liver neoplasms
- From:
Chinese Journal of Pathophysiology
2014;(12):2142-2147
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To investigate the effect of silencing cell division cycle 25a ( CDC25a) gene on the prolifera-tion of human hepatoma HepG2 cells.METHODS:CDC25a gene in human hepatoma HepG2 cells was silenced by RNA interference.Real-time PCR was applied to detect the expression of CDC25a, cyclin E and CDK2 at mRNA levels in the HepG2 cells.Western blotting was applied to detect the expression of CDC25a at protein level.In addition, MTT assay, Giemsa staining and flow cytometry were used to measure the proliferation of human hepatoma HepG2 cells.RESULTS:The expression of CDC25a at mRNA and protein levels in RNA silence group was lower than those in negative control group and normal control group (P<0.05).The mRNA expression of cyclin E and CDK2 in silence group was lower than that in negative control group and normal control group (P<0.05).The cell proliferation in silence group was lower than that in negative control group and normal control group ( P<0.05) .The results of flow cytometry revealed that the cells in silence group were blocked in G1 phase.CONCLUSION:Infection of LV-CDC25a-RNAi recombinant to the HepG2 cells effec-tively inhibits the CDC25a gene expression and the proliferation of human hepatoma cells, and arrests the cells in G1 phase, suggesting that CDC25a gene may be a key target for the treatment of liver cancer.
