Effect of insulin resistance on biological function of HepG2 cells and sen-sitivity to cisplatin

VernacularTitle:胰岛素抗性对肝癌HepG2细胞生物学功能及顺铂敏感性的影响
Author: Peng LIU ; Meisong HUANG ; Yangqian HU
Publication Type:Journal Article
Keywords: HepG2 cells; Insulin resistance; Apoptosis; Cell migration; Cisplatin
From: Chinese Journal of Pathophysiology 2014;(12):2148-2154
CountryChina
Language:Chinese
Abstract: [ ABSTRACT] AIM:To investigate the effect of insulin resistance ( IR) on the biological function of hepatocellu-lar carcinoma ( HCC) and sensitivity to cisplatin.METHODS:IR was induced in HepG2 cells via incubation with a high concentration of insulin.Afterwards, the effects of IR on adhesion, migration, invasion and sensitivity to cisplatin of the cells were detected.RESULTS:The results indicated that glucose consumption was reduced in the IR cells.The expres-sion of the insulin receptor and glucose transporter 2 was down-regulated.Furthermore, HepG2/IR cells displayed marked-ly enhanced adhesion, migration, and invasion.These cells exhibited a lower sensitivity to cisplatin.On the contrary, HepG2/IR cells exhibited decreased adhesion and invasion after treatment with the insulin sensitizer pioglitazone hydrochlo-ride.CONCLUSION:IR is closely related to drug resistance, adhesion, migration and invasion in HepG2 cells.These findings may help explain the clinical observation of the limited efficacy of chemotherapy on a background of IR.