Roles of transcription factor Bach1 in human microvascular endothelial cell function
10.3969/j.issn.1000-4718.2014.12.014
- VernacularTitle:转录因子Bach1对人微血管内皮细胞的作用研究
- Author:
Junxu LIU
;
Li JIANG
;
Xiangxiang WEI
;
Cong NIU
;
Sifeng CHEN
;
Dan MENG
- Publication Type:Journal Article
- Keywords:
Transcription factor Bach1;
Human microvascular endothelial cells;
Vascular endothelial growth factor;
Heme oxygenase-1;
Cell migration
- From:
Chinese Journal of Pathophysiology
2014;(12):2195-2200
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To determine the role of transcription factor Bach1 in the functions of human microvascular en-dothelial cells ( HMVECs ) .METHODS: Bach1 siRNA was transfected into HMVECs to knock down the expression of Bach1.In vitro endothelial cell tube formation assay in Matrigel culture was used as a surrogate assay for angiogenic poten-tial.Migration of HMVECs was determined by using Transwell chambers.Cell proliferation was measured by CCK-8 assay. Real-time PCR, Western blotting, and ELISA were employed to determine mRNA expression and protein level.Reporter as-say was performed to determine vascular endothelial growth factor ( VEGF) transcriptional activity.RESULTS:Knockdown of Bach1 expression in HMVECs led to an increase in the tube formation and increased endothelial cell migration ability, whereas it has little effect on cell proliferation.Bach1 silencing increased the mRNA and protein expression of heme oxygen-ase-1 (HO-1), and enhanced VEGF transcriptional activation, and mRNA and protein expression.CONCLUSION:Bach1 silencing increases HO-1 and VEGF expression, thus promoting the cell migration and tube formation of HMVECs, indicating that Bach1 is a repressor for angiogenesis.
