CD147 affects autophagy of prostate cancer PC-3 cells
10.3969/j.issn.1000-4718.2014.12.025
- VernacularTitle:CD147对前列腺癌PC-3细胞自噬的影响
- Author:
Fang FANG
;
Chun FENG
;
Yang YAO
;
Xiaoxiao MA
;
Liguo WANG
- Publication Type:Journal Article
- Keywords:
Antigens,CD147;
PC-3 cells;
Autophagy
- From:
Chinese Journal of Pathophysiology
2014;(12):2259-2261
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM: To study the autophagy of prostate cancer PC-3 cells induced by CD147 in vitro.ME-THODS:Themethod of amino acid starvation to induce autophagy was used.The expression of CD147 was detected by Western blotting.To study the functional effects of CD147 on autophagy in prostate cancer PC-3 cells, the down-regulation of CD147 expression was induced by the technique of RNAi.The conversion of autophagic marker protein LC3-I to LC3-II was determined by Western blotting.The cell death after starvation-induced autophagy was analyzed by trypan blue exclu-sion assay.RESULTS:The CD147 expression gradually increased in starvation-induced autophagy.The down-regulation of CD147 significantly increased the expression of autophagy-related protein LC3-II compared with control group.Mean-while, the cell death rates increased from (19.3 ±3.1)%and (22.3 ±3.5)%in control groups to (38.4 ±3.1)%in si-lencing the expression of CD147 in the PC-3 cells (P<0.05).CONCLUSION:CD147 inhibits starvation-induced auto-phgy and autophagy death in the prostate cancer PC-3 cells.
