Correlation of serum omentin-1 level and insulin resistance in rats
10.3969/j.issn.1000-4718.2014.12.029
- VernacularTitle:大鼠血清网膜素-1水平与胰岛素抵抗的相关性
- Author:
Nannan WANG
;
Xiaojuan ZHAO
;
Wei CHEN
;
Shuting BAI
;
Bowen YANG
- Publication Type:Journal Article
- Keywords:
Insulin resistance;
Hyperinsulinaemic-euglycaemic clamp;
Omentin-1
- From:
Chinese Journal of Pathophysiology
2014;(12):2276-2279
- CountryChina
- Language:Chinese
-
Abstract:
[ ABSTRACT] AIM:To establish the insulin resistance rat model for evaluating the correlation of omentin-1 level and insulin resistance.METHODS: SPF male Wistar rats ( n =30 ) were randomly divided into normal control group (NC, n=15) and high-fat diet group (HF, n=15).The rats in NC group were fed with basic diet.The insulin resistant model was established by feeding the rats with high-fat diet in HF group.After 10 weeks, 5 rats in each group were as-sessed by the technique of hyperinsulinaemic-euglycaemic clamp.After the insulin resistant model was successfully estab-lished, the body weight and fasting blood glucose were detected.The concentration of fasting serum omentin-1 was analyzed by ELISA.Fasting serum insulin was measured by radioimmunoassay.RESULTS: No difference of fasting blood glucose between the 2 groups was observed.The level of fasting serum insulin in HF group was significantly higher than that in NC group ( P <0.05 ) .The level of serum omentin-1 in HF group were significantly decreased compared with NC group (P<0.01).Pearson’s correlation analysis showed that negative correlations between serum omentin-1 and fasting serum insulin (r=-0.654,P<0.01), serum omentin-1 and free fatty acid (r=-0.446, P<0.05) was found.CONCLU-SION:In rats, serum omentin-1 level began to decrease at insulin resistance stage.As serum omentin-1 level decreased, the basal insulin level increased, indicating that decreased serum omentin-1 level may be an early factor of IR, diabetes and cardiovascular diseases.
