Effect of Dendrobium Nobile Lindl Decoction on Expression of PPARγin Diabetic Nephropathy Rat Kidney
10.3870∕yydb.2014.12.002
- VernacularTitle:金钗石斛水煎剂对糖尿病肾病大鼠肾脏PPARγ表达的影响
- Author:
Yuan_yuan LIU
;
Yan_lei ZHANG
;
Xiao_ran HE
;
Xiao_qiong LI
;
Yan_ping TANG
- Publication Type:Journal Article
- Keywords:
Dendrobium nobile lindl decoction;
NePhroPathy,diabetic;
Peroxisome Proliferator actiVated recePtor γ
- From:
Herald of Medicine
2014;(12):1545-1548
- CountryChina
- Language:Chinese
-
Abstract:
Objective To exPlore the ProtectiVe mechanism of Dendrobium nobile lindl ( DNL ) decoction on the exPression of Peroxisome Proliferator actiVated recePtor gamma ( PPARγ) in the renal cortex of diabetic nePhroPathy ( DN) rats. Methods Sixty SD rats were randomly diVided into six grouPs (n=10 Per grouP) as follows: normal control grouP (NC), diabetic nePhroPathy control grouP ( DN) ,rosiglitazone grouP ( RGZ) ,the Dendrobium nobile lindl low ( LD) ,medium ( MD) and high ( HD) dose grouPs. After DN rat model was established, the rats were administrated with resPectiVe medications for 12 weeks,resPectiVely. The renal Pathology of rats was obserVed. The mRNA and Protein exPression of PPARγ in the renal cortex were detected via Real_time PCR and Western blotting,resPectiVely. Results High dose DNL decoction significantly alleViated thickening of kidney tissue basement membrane and fusion of foot Process in model rats. The leVels of PPARγmRNA and Protein exPression in the MD and HD grouPs were significantly increased as comPared with the DN grouP (P<0. 05,P<0. 01). Conclusion DNL decoction can effectiVely reduce kidney injury by uP_regulating the PPARγ mRNA and Protein exPression in diabetic nePhroPathy rats.
