Effect of Different Sample Solvents on Determination of Astragaloside Ⅳ by High Performance Liquid Chromatograph_Evaporative Light Scattering Detector
10.3870∕yydb.2014.12.022
- VernacularTitle:不同样品溶剂对高效液相色谱_蒸发光散射检测器测定黄芪甲苷的影响
- Author:
Jing XU
;
Tianqiang LIU
;
Hengyang PENG
;
Dan XIAO
- Publication Type:Journal Article
- Keywords:
AstragaliⅣ;
SamPle_solVent;
SolVent effect;
ChromatograPh,high Performance liquid;
Detector,eVaPoratiVe light scattering
- From:
Herald of Medicine
2014;(12):1624-1627
- CountryChina
- Language:Chinese
-
Abstract:
Objective To exPlore the effect of samPle_solVent comPositions on the determination of AstragaliⅣby high Performance liquid chromatograPh_ eVaPoratiVe light scattering detector ( HPLC_ELSD ) . Methods Radix astragali and ganduqing granules serVed as samPles. Methanol,90%methanol,80%methanol,70%methanol,32%acetonitrile,15%acetonitrile, and water were samPle solVents. HPLC_ELSD was used to determine content of astragalosideⅣ. Results The results showed that the 90%methanol solution was an aPProPriate samPle solVent with good system suitability,Precision,accuracy,and,linearity etc. . Conclusion This method benefits the quality control of astragalosideⅣin Radix astragali and agents containing Radix astragali.
