Rat’s Schwann cells induce neural differentiation of human umbilical cord mesenchymal stem cells
10.3969/j.issn.2095-4344.2014.45.007
- VernacularTitle:人脐带间充质干细胞与乳鼠许旺细胞共培养向类神经元样细胞的分化
- Author:
Jiaxue ZHU
;
Hairong TAO
;
Zunli SHEN
- Publication Type:Journal Article
- Keywords:
umbilical cord;
mesenchymal stem cel s;
Schwann cel s;
cel culture techniques
- From:
Chinese Journal of Tissue Engineering Research
2014;(45):7250-7254
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Mesenchymal stem cel s can differentiate into nerve cel s by chemical induction or co-culture method, but whether mesenchymal stem cel s co-cultured with Schwann cel s differentiate into neuronal-like cel s or Schwann-like cel s is stil controversial.
OBJECTIVE:To explore the inductive role of Schwann cel s derived from rats in the differentiation of human umbilical cord mesenchymal stem cel s.
METHODS:Cocultures of human umbilical cord mesenchymal stem cel s (1×109/L) and Schwann cel s (1×109/L) from neonatal rats were performed using transwel culture dishes. After 2 weeks of cocultures, morphology of the cultured human umbilical cord mesenchymal stem cel s was observed, and the phenotypic changes of cel s were also detected with immumocytochemistry techniques.
RESULTS AND CONCLUSION:After 2 weeks of cocultures, some differentiated cel s showed neuron-like morphology, and expressed nestin, NF-200 andβ-III-tubulin, but did not express Schwann cel special marker S100 and oligodendrocytes special marker MAB1580. These findings indicate that human umbilical cord mesenchymal stem cel s can transdifferentiate into neuronal-like cel s by cocultures with rat’s Schwann cel s.
