Isolation and identification of neural stem cells from newborn mouse hippocampus, olfactory bulb and cortex
10.3969/j.issn.2095-4344.2014.45.010
- VernacularTitle:新生小鼠海马、嗅球及皮质神经干细胞的分离培养及鉴定
- Author:
Junning MA
;
Junwei GAO
;
Boru HOU
;
Haijun REN
;
Sihua CHEN
;
Jixing LIU
;
Guizhong YAN
- Publication Type:Journal Article
- Keywords:
neural stem cel s;
mice;
hippocampus;
olfactory bulb
- From:
Chinese Journal of Tissue Engineering Research
2014;(45):7266-7272
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:To in vitro isolate neural stem cel s with high purity and uniform biological properties and to establish a complete set of neural stem cel culture system is the basis for neural stem cel research.
OBJECTIVE:To establish an isolation and culture system for neural stem cel s from newborn mouse hippocampus, olfactory bulb and cortex and to analyze the biological properties of cel s.
METHODS:Neural stem cel s were isolated from the hippocampus, olfactory bulb and cortex tissue of newborn Kunming mice by mechanical separation and trypsin digestion. Serum-free culture technology, mechanical pipetting and trypsin digestion were used for subculture of neural stem cel s. 10%fetal bovine serum was used to induce differentiation of neural stem cel s. Neural stem cel s and their differentiated products were identified by
immunofluorescent staining of Nestin, CD133,β-TubulinIII, glial fibril ary acidic protein.
RESULTS AND CONCLUSION:The neural stem cel obtained from newborn mouse hippocampus, olfactory bulb and cortex had the capacity of self-renewal and differentiation which were positive for Nestin and CD133. After induction with fetal bovine serum, neural stem cel could differentiation toβ-tubulinIII or glial fibril ary acidic protein positive cel s that were neurons and astrocytes. This experiment has successful y established the neural stem cel isolation, culture, identification and induction system, providing experimental basis for subsequent studies of neural stem cel s.
