Effect of Emodin on Hypertrophic Scar Fibroblasts
10.3870∕yydb.2014.12.007
- VernacularTitle:大黄素对增生性瘢痕成纤维细胞的作用
- Author:
Yilun LIU
;
Fengmei DENG
;
Weihua LIU
;
Yonghui LUO
;
Ningning ZHAO
;
Hairong LIU
;
Yueming LIU
;
Hangyu WANG
- Publication Type:Journal Article
- Keywords:
Emodin;
Scar,hyPertroPhic;
Fibroblast;
Cell death
- From:
Herald of Medicine
2014;(12):1566-1570
- CountryChina
- Language:Chinese
-
Abstract:
Objective To inVestigate the effect of emodin on hyPertroPhic scar fibroblasts ( HSFs ) and exPlore the underlying mechanism. Methods HSFs were treated by emodin at final concentrations of 0,20,40,and 80 μmol·L-1, resPectiVely,in the cultural media. Forty_eight hours later,the cells were subjected to MTS assay and flow cytometry assay with annexin V and ProPidium iodide as dyeing indicators. Whole cell lysates from the cells of eVery grouP were subjected to Western blotting to measure the Protein exPression leVels of ERK1∕2,Bcl_2,Mcl_1 and RIP1. Results The cell Viability of HSFs was inhibited by emodin in a dose dePendent manner. The mortality rate of HSFs treated with emodin for 48 h at the concentrations of 40 and 80 μmol·L-1 were 28. 6%and 68. 0%,resPectiVely,which was significantly higher than that of the control grouP ( P<0.01).Pretreatmentwith Z_VAD_FMK could Partially reduce the mortality caused by emodin (P<0.05).PhosPhorylation of ERK1∕2 and the exPression of RIP1 and Mcl_1 were inhibited by emodin. Conclusion Down regulation of ERK1∕2,RIP1 and Mcl_1 by emodin may account for the inhibited Proliferation and increased cell death of HSFs.
