Expansion of chicken bone marrow mesenchymal stem cells by laminin culture system
10.3969/j.issn.2095-4344.2014.45.002
- VernacularTitle:利用层粘连蛋白扩增罗曼鹤鸡骨髓间充质干细胞
- Author:
Shuangxing LI
;
Yuan QI
;
Fengyuan PIAO
;
Yachen LI
;
Xiaohui LIU
;
Jing SHAO
;
Shuangyue LI
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cel s;
laminin;
extracel ular matrix;
cel proliferation;
chickens
- From:
Chinese Journal of Tissue Engineering Research
2014;(45):7222-7226
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Bone marrow mesenchymal stem cel s from chickens are important cel models for embryonic developmental biology, immunology and oncology research. However, it is difficult to keep bone marrow mesenchymal stem cel s with good undifferentiated potential in a large-scale expansion system.
OBJECTIVE:To establish a culture system in vitro with laminin coating to expand bone marrow mesenchymal stem cel s from chickens.
METHODS:Isolated bone marrow mesenchymal stem cel s from chickens were seeded in laminin-coated plates and traditional two-dimensional plates, respectively. After expansion in vitro, the morphological characteristics, expression of surface markers, expansion characteristics and adipogenic differentiation of bone marrow mesenchymal stem cel s in both conditions were analyzed and compared.
RESULTS AND CONCLUSION:There were no statistical differences in the morphological characteristics and expression of surface markers of bone marrow mesenchymal stem cel s expanded by laminin-coated plates and traditional two-dimensional plates. But, the expansion characteristics and adipogenic differentiation of bone marrow mesenchymal stem cel s cultured in laminin-coated plates were better than those in traditional two-dimensional plates. Laminin culture system could quickly amplify out of a large number of chicken bone marrow mesenchymal stem cel s with better proliferation ability and undifferentiated performance. Al above results indicated that a more efficient expansion system with laminin coating is established.
