Research on the determination of molecular weight distribution and the peptide content of Ganlong capsule
- VernacularTitle:肝龙胶囊中总肽含量及其分子量分布的研究
- Author:
Kaige NA
;
Hongxia MAN
;
Qiaoyun TAN
;
Yongshou YANG
;
Peiyun XIAO
- Publication Type:Journal Article
- Keywords:
Ganlong capsule;
size exclusion chromatography;
peptide content;
molecular weight distribution
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(3):159-161
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the molecular weight distribution of Ganlong capsule by HPSEC the content of the peptide determined by Lowry and Methods The superdex peptide 10/300 GL (10 mm ×300 mm) column was used.The pH=6.0 and phosphate buffer of 0.05 mol/L was used as the mobile phase, containing 0.1 mol/L NaCl.The flow rate was set at 0.7 mL/min;The column temperature was 25℃;The detection wavelength was 214 nm.Results The content of the peptide ranged from 0.08 mg to 0.4 mg ( r =0.9996 ) .The RSDs of measurement precision of molecular weight and content were 0.08% and 0%(n=6), respectively.The RSDs of the repeatability were 1.3% and 1.1%(n=6);The regression equation of standard material was logMr =5.1455 -0.0871tR, r =0.9983,the relative molecular weight ranged from 2.68 ×102 Da ~5.73 ×103 Da(r =0.9983). Conclusion on The method is simple and rapid for determining the peptide content and the molecular weight distribution of Ganlong capsule.It can be used quality control method for Ganlong capsule.
