Effect of Transcutaneous Acupoint Electrical Stimulation on Oocyte and Bcl-2 and Bax in the Older Women
10.13460/j.issn.1005-0957.2014.12.1097
- VernacularTitle:经皮穴位电刺激对高龄妇女卵细胞质量及Bcl-2、Bax的影响
- Author:
Fang LIAN
;
Fei LIU
;
Zhenhong SHUAI
- Publication Type:Journal Article
- Keywords:
Transcutaneous acupoint electrical stimulation;
Bcl-2;
Bax;
Oocyte quality;
In vitro fertilization-embryo transfer
- From:
Shanghai Journal of Acupuncture and Moxibustion
2014;(12):1097-1099
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo observe the effect of transcutaneous acupoint electrical stimulation (TAES) on the ovariangranulosa cell apoptosis-related proteins, Bcl-2 and Bax, as well as the oocyte quality in theolder women with kidney deficiency receiving in vitro fertilization-embryo transfer (IVF-ET).MethodSixty-six older women with kidney deficiency who were receiving IVF-ET because of the fallopian tube factor were randomized into a treatment group (intervened by TAES) and a control group (intervened by sham acupuncture), 33 in each group. The kidney-deficiency syndrome score, HCG E2/follicle count per day, excellent follicle rate, excellent embryo rate, and clinical pregnancy rate were observed and compared between the two groups; Western blot was used to detect the expression of protein Bcl-2 and Bax.ResultCompared to the control group, the treatment group had significant improvement in kidney-deficiencysymptoms (P<0.01), significant increases in theHCG E2/follicle count per day, excellent follicle rate, and excellent embryo rate (P<0.05), enhanced expression of Bcl-2 protein (P<0.01), and decreased expression of Bax protein (P<0.01); while there were no significant differences in comparing theretrievedoocyte number and clinical pregnancy rate (P>0.05). The expression of Bcl-2 and Bax proteins were markedlycorrelatedwith IVF-related indexes (P<0.05).ConclusionTAES can improve the kidney-deficiency symptoms and the quality of oocyte and embryo inolder women; this action is possibly related to the regulation of expression of the cell apoptosis-related protein Bcl-2 and Bax, as well as the decrease ofgranulosecell apoptosis.
