Comparison of the chemical composition between fresh and dried venenum bufonis by UPLC-Orbitrap MS.
- Author:
Jing HE
;
Yan LI
;
Nan SI
;
Haiyu ZHAO
;
Baolin BIAN
;
Hongjie WANG
- Publication Type:Journal Article
- From:
Acta Pharmaceutica Sinica
2014;49(10):1446-50
- CountryChina
- Language:Chinese
-
Abstract:
To identify the active components in Bufo melanostictus Schneider and clarify the difference between fresh and dried Venenum Bufonis, a UPLC-Orbitrap MS method has been established. The separation was performed with gradient elution of acetonitrile and water (with 0.1% formic acid) as mobile phase. By comparing their retention time and high resolution mass data of Venenum Bufonis extracts, 39 effective components were primarily identified by MS/MS analysis in positive ion mode. Twenty-six of them were bufadienolides. There were significant differences in the main composition between fresh and dried Venenum Bufonis. There are fewer bufadienolides in fresh toad venom.
