Investigation of microRNA-373 function and mechanism in HepG2 cell growth suppression induced by 5-fluorouracil
10.3760/cma.j.issn.1007-8118.2014.09.014
- VernacularTitle:microRNA-373在5-氟尿嘧啶抑制HepG2细胞增殖中的功能及作用机制研究
- Author:
Ziwen LIU
;
Yongxing DU
;
Lei YOU
;
Hong SHU
;
Taiping ZHANG
;
Yupei ZHAO
- Publication Type:Journal Article
- Keywords:
5-fluorouracil;
Hepatocellular carcinoma;
microRNA-373;
Cell growth
- From:
Chinese Journal of Hepatobiliary Surgery
2014;20(9):675-678
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of 5-fluorouracil (5-FU) on hepatocellular carcinoma (HCC) cell growth and to elucidate its potential molecular mechanism.Methods Real-time PCR analysis was conducted to determine the expression of miR-373 in HCC tissue specimens and HCC cell lines.The expression of miR-373 was also evaluated in HepG2 cells after 5-FU treatment.Western blot analysis was performed to detect the protein levels of PPP6C,a verified target of miR-373,with transfection of miR-373 mimics or 5-FU treatment.A rescue assay was conducted to investigate the cell growth in HepG2 cells by using CCK-8.Results miR-373 expression was up-regulated in both HCC tissues and cell lines.miR-373 expression depicted about 2.94-fold augment in HepG2 cells as compared to normal liver cells control (P ＜0.01).5-FU treatment led to a significant decrease of miR-373 levels (approximately 50％,P ＜0.01,48 h) and resulted in a marked increase of PPP6C protein (approximately 2.1-fold,48 h) in HepG2 cells.The overexpression of miR-373 could prevent the impact of 5-FU treatment on cell growth in HepG2 cells and CCK-8 assay showed that HepG2 cell growth was rescued approximately 81％ and 84％ at 24 h (P ＜ 0.05) and 48 h (P ＜ 0.01),respectively.Conclusion 5-FU can repress endogenous miR-373 level,which activates the expression of downstream targeted gene PPP6C,thereby exerting an inhibitory effect on HepG2 cells.
