Effects of Rosuvastatin and Losartan on expression of caveolin-1 in cultured human monocyte-macrophage cells induced by oxidized low density lipoprotein
10.3760/cma.j.issn.1008-1372.2014.08.013
- VernacularTitle:瑞舒伐他汀联合氯沙坦对ox-LDL诱导的人单核巨噬细胞小凹蛋白-1表达的影响
- Author:
Yubin HE
;
Lina LIU
;
Zheng WEI
;
Pengjuan JI
;
Xiaoyu SHEN
- Publication Type:Journal Article
- Keywords:
Antilipemic agents/pharmacology;
Fluorobenzenes/pharmacology;
Pyrimidines/pharmacology;
Sulfonamides/pharmacology;
Biphenyl compounds/pharmacology;
Tetrazoles/pharmacology;
Imidazoles/pharmacology;
Lipoproteins,LDL;
Mononuclear phagocyte system/dr
- From:
Journal of Chinese Physician
2014;16(8):1052-1055
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects,mechanisms,and the optimum doses of Rosuvastatin and Losartan on expression of caveolin-1 in cultured human monocyte-macrophage cells which were induced by oxidized low density lipoprotein(ox-LDL).Methods Human-monocyte cells were separated and changed into the human monocyte-macrophage cells.The model of amerosclerosis was set up.These cells were incubated in different doses of Rosuvastatin(0.1,1.0,5.0 μmol/L) and Losartan (10,50,100 μmol/L),and then cultured in combination of two drags (5.0 μmol/L + 100 μmol/L).Expression of caveolin-1 mRNA was determined with real-time fluorescent quantitative polymerase chain reaction (RT-PCR).Results In ox-LDL group,caveolin-1 mRNA was decreased sharply relative to control group [(0.2533 ±0.00973) vs (0.9410 ±0.03677)] in a concentration-dependent manner (P ＜0.01).Compared to ox-LDL group,expressions of Caveolin-1 mRNA were increased gradually in different doses of Rosuvastatin alone and Losartan alone group [(0.5198 ± 0.04840),(0.6183 ± 0.06740),(0.7257 ± 0.03052) vs (0.2533 ± 0.00973) ; (0.3350 ± 0.04177),(0.4428 ± 0.03804),(0.6049 ± 0.02627) vs (0.2533 ± 0.00973)] in a concentration-dependent manner (P ＜ 0.01) ; the summit expressions of caveolin-1 mRNA were emerged in using Rosuvastatin and Losartan together (F =59.119,P ＜ 0.01).Conclusions Rosuvastatin and Losartan may be responsible for the expression of caveolin-1 in human monocyte-macrophage cells that were induced by ox-LDL.The expressions were up-regulated with dose dependent manner of these drugs,and got the crest stage when using optimum doses of Rosuvastatin and Losartan together.
