The effect of heat stress on the permeability, cytoskeleton and cell cycle of human skeletal muscle cell
10.3760/cma.j.issn.1671-0282.2014.08.008
- VernacularTitle:热打击对人骨骼肌细胞通透性、细胞骨架及细胞周期的影响
- Author:
Zhiguo PAN
;
Yu SHAO
;
Jun WAN
;
Yan GENG
;
Jinghe CHEN
;
Lei SU
- Publication Type:Journal Article
- Keywords:
Heat stress;
Skeletal muscle cells;
Permeability;
Calcium overload;
Cytoskeleton;
Cell cycles;
Coomassie brilliant blue;
Coomassie brilliant blue;
Flow cytometry
- From:
Chinese Journal of Emergency Medicine
2014;23(8):862-865
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of heat stress on the permeability,cytoskeleton and cell cycle of human skeletal muscle cell (HSKMC).Methods The HSKMC membrane permeability was detected by calcium ion inflow with flow cytometer,the cytoskeleton was stained by CBB 250,and the cell cycle was determined by flow cytometer.Results After 1 h of heat stress on human HSKMC cells under different temperature gradient,the median level of calcium ion was 91.63 in 43 ℃ heat stress group compared with 22.98 in 37 ℃ control group.As temperature increased,thicker and shorter cytoskeleton and stress fiber were shown under the high power lens of microscope.The DNA expression of skeleton cells at G0/G1 stage was 44.13-62.98 in groups under heat stress.Compared with normal control group,DNA expression was much higher in heat stress group,when HSKMC was cultured under 37 ℃ temperature for another 18 h,it kept decreasing DNA expression to a similar level as control group.Conclusions Heat stress can cause calcium iron inflow resulting in intracellular calcium overload,and affect the cytoskeleton leading to loss of normal web ordered arrangement and increased gap in HSKMC cells,which give rise to blocking cell cycle into G0/G1 stage.
