Changes of mucosa associated bacterial flora in patients with ulcerative colitis at different stages and its significance
- VernacularTitle:不同分期溃疡性结肠炎患者黏膜相关菌群的变化及意义
- Author:
Yuan CHU
;
Qiaomin WANG
- Publication Type:Journal Article
- Keywords:
ulcerative colitis;
mucosa associated bacterial flora;
real-time PCR
- From:
Acta Universitatis Medicinalis Anhui
2014;(10):1444-1447
- CountryChina
- Language:Chinese
-
Abstract:
Objective Sigmoid mucosa specimens of the patients with ulcerative colitis ( UC ) at active stage and remission stage were respectively detected by real-time PCR for the contents of the six kinds of bacterial floras inclu-ding fusobacterium, enterococcus, lactobacillus, bifidobacterium, bacteroides, and escherichia coli. So the possi-ble roles and significance of the changes of intestinal mucosa associated bacterial flora in the pathogenesis of UC were discussed. Methods Sigmoid biopsy tissues were collected from 35 UC patients ( 20 cases were activities group while 15 cases were remission group) and 20 healthy cases( control group) . Specific primers were set accord-ing to the bacterial 16 SrDNA sequences. Bacterial DNA of the intestinal mucosa specimens was extracted, and re-al-time PCR was used to detect the numbers of different bacterial colonies. Results In sigmoid mucosa specimens of the UC group at activities group, escherichia coli colony was increased, while bifidobacterium, bacteroides, lac-tobacillus and fusobacterium, were reduced compared to the control group(P<0.05). But for enterococcus, there was no significant change(P>0.05). And in remission group, bacteroides and bifidobacterium were reduced com-pared with the control group(P<0.05), while no significant changes were found in escherichia coli, lactobacillus, fusobacterium and enterococcus( P >0.05 ) . The ration of bifidobacterium to escherichia coli ( B/E ) in UC pa-tients at active stage was less than 1, which was lower than the control group. While B/E values in UC patients at remission stage and the control group were both larger than 1 , with no statistically significant difference between them. Conclusion There were obvious changes of intestinal bacterial flora in UC patients, and the change is more obvious in the UC patients at active stage, showing that there is a close relationship between intestinal mucosa asso-ciated bacterial flora and the development of UC.
