Inhibition of c-Myc by 10058-F4 overcomes imatinib resistance in chronic myeloid leukemia cells
10.3969/j.issn.1000-4718.2014.09.009
- VernacularTitle:c-M yc 抑制剂10058-F4对伊马替尼耐药细胞的增殖抑制作用
- Author:
Zijie LONG
;
Zhigang FANG
;
Xiaona PAN
;
Ruifang FAN
;
Dongjun LIN
- Publication Type:Journal Article
- Keywords:
c-Myc;
10058-F4;
Imatinib resistance;
Chronic myeloid leukemia
- From:
Chinese Journal of Pathophysiology
2014;(9):1590-1594
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of c-Myc inhibitor 10058-F4 on human chronic myeloid leukemia ( CML) K562 cells and imatinib-resistant K562/G cells.METHODS: The protein expression of c-Myc was detected by Western blotting .Cell proliferation was evaluated by MTT assay and colony formation assay .PI staining was used to deter-mine the cell cycle distribution .Annexin V-PI staining was applied for apoptosis detection .RESULTS:Imatinib-resistant K562/G cells displayed lower sensitivity to imatinib than K 562 cells with high expression of c-Myc.Treatment with specific c-Myc inhibitor 10058-F4 inhibited the cell proliferation in a dose-and time-dependent manner , and K562/G displayed more sensitivity to 10058-F4 than K562 cells.10058-F4 also induced cell cycle arrest in G 0/G1 phase and induced apoptot-ic cell death in the 2 cells.Importantly, 10058-F4 suppressed the colony formation ability in K 562 and K562/G cells. CONCLUSION:c-Myc is a novel target to overcome imatinib-induced drug resistance , and c-Myc inhibitor provides a new approach in CML therapy .