Influence of high-mobility group box 1 on proliferation of neural stem cell in pre-infarction cortex of focal cerebral ischemia/reperfusion model rats
10.3969/j.issn.1000-4718.2014.09.013
- VernacularTitle:高迁移率族蛋白 B1对大鼠局灶脑缺血／再灌注后大脑皮质梗死周围区神经干细胞增殖的影响
- Author:
Man LI
;
Yong LUO
;
Yuan LI
;
Lin SUN
- Publication Type:Journal Article
- Keywords:
Focal cerebral ischemia/reperfusion;
High-mobility group box 1;
Neural stem cells;
RNA inter-ference;
Lentivirus
- From:
Chinese Journal of Pathophysiology
2014;(9):1616-1621
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the influence of high-mobility group box 1 (HMGB1) on the proliferation of neural stem cells in peri-infarction cortex of focal cerebral ischemia/reperfusion model rats .METHODS: Male SD rats (n=48) were randomly divided into sham group , ischemia/reperfusion (I/R) group, RNA interference group and nega-tive interference group .The rat middle cerebral artery was blocked to establish focal cerebral I /R model ( ischemia for 1 h and reperfusion for 7 d).Lentivirus vector of HMGB1 shRNA was used to suppress the HMGB1 protein expression in the rat brain.The effect of RNA interference was evaluated by the methods of double-immunofluorescence labeling of HMGB 1/GFAP and Western blotting .The proliferation of neural stem cells in the peri-infarction cortex was assessed by double labe-ling of BrdU/nestin.RESULTS: The protein expression of HMGB1 in I/R group was much higher than those in sham group (P<0.05).RNA interference effectively inhibited the HMGB1 expression (P<0.05).Double labeled BrdU/nestin positive cells in I/R group were more than that in sham group (P<0.05).The double labeled BrdU/nestin positive cells were significantly decreased in RNA interference group (P<0.05).CONCLUSION:Focal cerebral ischemia/reperfusion injury promotes the proliferation of neural stem cells in peri-infarction cortex by increasing HMGB 1 protein level .
