Effects of Kv1.5 on proliferation and apoptosis of rat PASMCs under hy-poxia+hypercapnia condition and relationship with MAPK pathway
10.3969/j.issn.1000-4718.2014.09.017
- VernacularTitle:Kv1．5对大鼠低氧高二氧化碳性 PASMCs 增殖、凋亡的影响及与 MAPK 通路的关系
- Author:
Yingchun MA
;
Mengxiao ZHENG
;
Linjing HUANG
;
Yuanyuan WANG
;
Lei YING
;
Wantie WANG
- Publication Type:Journal Article
- Keywords:
Potassium channels;
Mitogen-activated protein kinase;
Pulmonary artery smooth muscle cells;
Cell proliferation;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2014;(9):1645-1650
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of voltage-dependent K +channel 1.5 (Kv1.5) on the proliferation and apoptosis of rat pulmonary artery smooth muscle cells (PASMCs) under hypoxia+hypercapnia condition and the relation-ship with mitogen-activated protein kinase(MAPK) signal pathway.METHODS:The PASMCs isolated from the male SD rat were cultured under hypoxia +hypercapnia condition, and randomly divided into normal group (N group), hypoxia+hyper-capnia group (HH group), hypoxia+hypercapnia+DMSO incubation group (HD group), hypoxia+hypercapnia +U0126 (an extracellular signal-regulated kinase 1/2 inhibitor) incubation group (HU group), hypoxia+hypercapnia+SB203580 (a p38 mitogen-activated protein kinase inhibitor ) incubation group (HS group), and hypoxia+hypercapnia+anisomycin (an agonist of MAPK) incubation group (HA group).Cell Counting Kit-8 was used to detect the cell viability.The protein expression of Kv1.5, PCNA and Bax was detected by Western blotting .RESULTS:Compared with N group , the cell via-bility and PCNA protein expression in HH group and HD group were significantly raised (P<0.01), but Kv1.5 and Bax proteins were significantly decreased (P<0.01).No difference between HH group and HD group was observed (P>0.05 ) .Compared with HD group , the cell viability and PCNA protein expression in HU group , HS group and HA group were decreased (P<0.05 or P<0.01), but Kv1.5 protein and Bax protein were raised (P<0.01), with the most significant changes in HA group .CONCLUSION:The regulation of Kv1.5 to the proliferation and apoptosis of PASMCs under hy-poxia+hypercapnia condition might have a relationship with the activation of MAPK signal pathway .
