Expression of micro RNA-29a and 133a in myocardium of atrium of per-sistent atrial fibrillation model dog
10.3969/j.issn.1000-4718.2014.09.022
- VernacularTitle:MicroRNA-29a、-133a在持续性房颤模型犬心房肌中的表达变化
- Author:
Fapeng LI
;
Jian ZHANG
;
Guojun XU
;
Tianyi GAN
;
Baopeng TANG
;
Yaodong LI
;
Ting MAO
;
Tao JIANG
- Publication Type:Journal Article
- Keywords:
Atrial fibrillation;
MicroRNAs;
Fibrosis;
Structural remodeling
- From:
Chinese Journal of Pathophysiology
2014;(9):1672-1676
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of microRNA-29a and 133a expression in the atrium on atrial fibril-lation (AF) and fibrosis.METHODS:Chronic rapid atrial pacing was used to establish the persistent AF dog model , and the sham group was also set up .The cardiac ultrasound measurement was used for determining the cardiac structure size . The Masson 3 color staining were used to evaluate the stage of fibrosis .The expression of microRNA-29a and 133a in the left atrium ( LA) was detected by real-time transcriptase polymerase chain reaction .RESULTS: Compared with before modeling , no statistical difference of atrial dilatation and decreased ejection fraction in the model dogs with persistent AF was observed (P>0.05).Compared with sham group, the degree of fibrosis and collagen volume fraction (CVF) in per-sistent AF model group were increased obviously (P<0.05).The expression of microRNA-29a and 133a were decreased obviously (P<0.01, P<0.05).CONCLUSION:Structural remodeling of the atrium and atrial fibrosis are the essential for development and maintenance of atrial fibrillation .Down-regulation of microRNA-29a and 133a expression may be very important molecular mechanism for atrial structural remodeling in the persistent AF model dogs .
